https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007 en-us Our small-scale bioreactor with a gas-permeable membrane has previously been shown to allow direct oxygenation of primary hepatocytes in collagenous sandwich cultures. In this work the applicability of this system for studying the response of hepatocytes to different ambient oxygen concentrations above the normoxic situation and the effect of the antioxidant vitamin C (ASC) on hepatocyte functionality in different oxygen cultures were evaluated. Determination of the parameters for functional levels (albumin and urea synthesis, biotransformation) combined with the model calculation of these parameters using a product inhibition model based on the concentration of hydrogen peroxide (H2 O2 ) indicated a relevance of oxygen levels for the maintenance of hepatic-specific functions. A model for the dynamics of oxidative stress was proposed to predict the time course of the qualitative concentrations of H2 O2 and the superoxide radical (O2.- ) in different oxygen cultures. The 20% and 30% O2 cultures exhibited similar differentiated hepatic functions that were unequivocally lost in the 40% O2 cultures apparently due to oxidative damage. Metabolic functions in the 20% and 30% O2 cultures could be improved by ASC. These findings suggest that our bioreactor model not only allows a prediction of the liver response to variations in oxygen environments, but also serves as an in vitro tool to screen new compounds for antioxidant capacity. https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007/@@file/0480d653700d274a4216023e9f45f532f1beb0b1 Merge Tommy Yu 2015-02-12 https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007/@@file/b79c9b42d6844f0c29e292544e22c38f16f47f3e Added xul and session file for a model, does not reproduce published results so have not made b and c versions yet Hanne Nielsen 2011-09-29 https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007/@@file/46dcfe96875c053671d2985a8f6b80eb88f627f1 Edited HTML file Hanne Nielsen 2011-09-02 https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007/@@file/8e607253f76e82ec87faea34b780290623207acc Added HTML file Hanne Nielsen 2011-08-19 https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007/@@file/9499f3061b2ab318d312420c9635368a30cb63d7 Added HTML file Hanne Nielsen 2011-08-19 https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007/@@file/566a74a942fccb1caec0e3e94f516e992a9f891a Removed build image and ds store Tommy Yu 2011-04-26 https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007/@@file/3282551decce1994dc061c5a67d1765cf078e547 Removed working ai file Hanne Nielsen 2011-04-15 https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007/@@file/4b74994cbe38a4e4d673b23fb5301dbce9846958 Added images in ai svg and png format Hanne Nielsen 2011-04-15 https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007/@@file/3a2274fda156f157c1d1a1e858dbdc3b20ac4305 Added documentation to CellML file. This file is for 20% oxygen, +ASC. Does not reproduce results in papers. Hanne Nielsen 2011-03-11 https://staging.physiomeproject.org/w/hnielsen/schmitmeier_2007/@@file/343e38b68ea73e8378abfe89a1a101992dce16d1 Added CellML file. Runs in OpenCell but does not reproduce results exactly. Hanne Nielsen 2011-03-11