• State: Published

Two compartment model of diazepam biotransformation in an organotypical culture of primary human hepatocytes

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Drug biotransformation is one of the most important parameters of preclinical screening tests for the registration of new drug candidates. Conventional existing tests rely on nonhuman models which deliver an incomplete metabolic profile of drugs due to the lack of proper CYP450 expression as seen in human liver in vivo. In order to overcome this limitation, we used an organotypical model of human primary hepatocytes for the biotransformation of the drug diazepam with special reference to metabolites in both the cell matrix phase and supernatant and its interaction of three inducers (phenobarbital, dexamethasone, aroclor 1254) in different time responses (1, 2, 4, 8, 24 h). This model runs in OpenCell to reproduce the results in figure 3 of the published paper. There are four model versions: control (a), aroclor (b), dexamethasone (c) and phenobarbital (d). Initial values were taken from the published graphs.
Hanne Nielsen <>
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Filename Size Date Options
.DS_Store 6148 2011-08-23 [browse] 97985 2011-08-23 [browse]
acikgoz_2009.html 2029 2011-08-23 [browse]
acikgoz_2009.png 40499 2011-08-23 [browse]
acikgoz_2009.svg 12731 2011-08-23 [browse]
acikgoz_2009.xul 21215 2011-08-23 [browse]
acikgoz_2009a.cellml 33702 2011-08-23 [browse]
acikgoz_2009a.session.xml 21994 2011-08-23 [browse] [run]
acikgoz_2009b.cellml 33705 2011-08-23 [browse]
acikgoz_2009b.session.xml 21343 2011-08-23 [browse] [run]
acikgoz_2009c.cellml 33711 2011-08-23 [browse]
acikgoz_2009c.session.xml 21341 2011-08-23 [browse] [run]
acikgoz_2009d.cellml 33710 2011-08-23 [browse]
acikgoz_2009d.session.xml 21343 2011-08-23 [browse] [run]